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The tender-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence employing a several sequence alignment (MSA) presented in the seqAn library [27]. Wham applies three filters to your consensus sequences. Breakpoints are usually not noted in situations where consensus sequences are shorter than 10 bp or consist
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Wham clusters the many prospect breakpoints and rounds their positions to the nearest tenth foundation pair. The posture with the highest study support is noted. In the event the smooth-clipped consensus sequence is often aligned into the putative breakpoint region utilizing the Smith-Waterman algorithm, the breakpoint is further more refined to th